Pathogenic bacteria fight versus oxidative stress

cell host microbe proposal h2o2 bacterial proteome cover 3dciencia web

RNA Polymerase II – TFIIB complex

Base resolution sequencing 5-hydroxymethylcytosine cell cover proposal

The study of 5-hydroxylmethylcytosines (5hmC) has been hampered by the lack of a method to map it at single-base resolution on a genome-wide scale. Affinity purification-based methods cannot precisely locate 5hmC nor accurately determine its relative abundance at each modified site. We here present a genome-wide approach, Tet-assisted bisulfite sequencing (TAB-Seq), that when combined with traditional bisulfite sequencing can be used for mapping 5hmC at base resolution and quantifying the relative abundance of 5hmC as well as 5mC. Application of this method to embryonic stem cells not only confirms widespread distribution of 5hmC in the mammalian genome but also reveals sequence bias and strand asymmetry at 5hmC sites. We observe high levels of 5hmC and reciprocally low levels of 5mC near but not on transcription factor-binding sites. Additionally, the relative abundance of 5hmC varies significantly among distinct functional sequence elements, suggesting different mechanisms for 5hmC deposition and maintenance.

Adenovirus icosahedral nucleocapsid structure

UHRF1 SRA domain bound with hemi-methylated CpG

Signal transducer and activator of transcription 5. STAT5

Human Papilloma dsDNA virus cutaway

Repression of Interferon Response Elements by NF-kB p50

The bacteriophage phi 29 DNA polymerase

PPAR gamma RXR bound to DNA