ketamine blockade of Pentameric ligand-gated ion channel

ketamine blockade of Pentameric ligand-gated ion channel

West Nile Virus. Encephalitis

[caption id="attachment_634" align="alignnone" width="807"] West Nile virus (WNV) is a mosquito-borne zoonotic arbovirus belonging to the genus Flavivirus in the family Flaviviridae.[/caption]

Base resolution sequencing 5-hydroxymethylcytosine cell cover proposal

The study of 5-hydroxylmethylcytosines (5hmC) has been hampered by the lack of a method to map it at single-base resolution on a genome-wide scale. Affinity purification-based methods cannot precisely locate 5hmC nor accurately determine its relative abundance at each modified site. We here present a genome-wide approach, Tet-assisted bisulfite sequencing (TAB-Seq), that when combined with traditional bisulfite sequencing can be used for mapping 5hmC at base resolution and quantifying the relative abundance of 5hmC as well as 5mC. Application of this method to embryonic stem cells not only confirms widespread distribution of 5hmC in the mammalian genome but also reveals sequence bias and strand asymmetry at 5hmC sites. We observe high levels of 5hmC and reciprocally low levels of 5mC near but not on transcription factor-binding sites. Additionally, the relative abundance of 5hmC varies significantly among distinct functional sequence elements, suggesting different mechanisms for 5hmC deposition and maintenance.

Similar structures distinct functions. From fatty acid carrier protein to Chalcone isomerase.

Como se ve en la figura el plegamiento tridimensional de la chalcona isomerasa y la proteína FAP es muy parecido. El sitio de unión para ácidos grasos se convirtió en el centro activo estéreo específico del enzima. Es un espectacular ejemplo evolutivo de la diversificación de las proteínas con el tiempo variando lo ya conocido. La chalcona isomerasa tiene unido en su centro la molécula de naringenina (un flavonoide muy presente en el pomelo). La FAP tiene unidas dos moléculas del ácido graso laurico.

Jupiter cut-away

Nature Chemical Biology. Human Thymine DNA glycosylase specifically recognizes 5-carboxylcytosine-modified DNA

Coagulation factor VIII

Fibrinogen structure

Crystal structure of fibrinogen. The central nodule, formed by the N-terminal portions of all six chains, is connected to the distal β- and γ-nodules formed by the C-terminal portions of the Bβ and γ chains, respectively, by triple-helical coiled-coils, each formed by the middle portions of the Aα, Bβ and γ chains.

Hemoglobin´s heme group bound to oxygen molecule

hemoglobin heme oxy haemoglobin structure hemoglobina hemo oxygen oxigeno histidine hitidina imadozole imidazol 3dc

Crystal Structure of Cholesteryl Ester Transfer Protein CETP